| 重组降血压肽多肽序列的设计及表达系统构建 |
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| 引用本文： | 刘冬,李世敏,张丽君,唐语谦,梁世中.重组降血压肽多肽序列的设计及表达系统构建[J].华南理工大学学报(自然科学版),2005,33(10):90-92. |
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| 作者姓名： | 刘冬 李世敏 张丽君 唐语谦 梁世中 |
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| 作者单位： | 华南理工大学,生物科学与工程学院,广东,广州,510640;深圳职业技术学院,应用化学与生物技术学院,广东,深圳,518055 |
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| 基金项目： | 广东省社会发展攻关资助项目（20032979）;深圳市科技计划资助项目（03KJ6021） |
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| 摘 要： | 通过分析降血压肽结构与功能的关系，设计并合成5条降血压肽序列，通过活性测定以及体外消化试验，确定P3（六肽）为理想降血压肽，为实现该小肽在大肠杆菌中的表达，经特定酶切位点将其串联为6拷贝的融合多肽，将相应的基因序列克隆至表达载体pGEX-4T-2并转化至宿主茵Escherichia coli BL21中，双酶切、PCR以及测序结果均表明成功构建了重组质粒pGEX-4T-2-ACEIP。
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| 关 键 词： | 降血压肽 序列设计 基因重组 |
| 文章编号： | 1000-565X（2005）10-0090-03 |
| 收稿时间： | 2004-12-08 |
| 修稿时间： | 2004年12月8日 |
Design of Recombined Angiotensin-Converting Enzyme Inhibitor Peptide Sequence and Construction of Expression System |
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| Liu Dong,Li Shi-min,Zhang Li-jun,Tang Yu-qian,Liang Shi-zhong.Design of Recombined Angiotensin-Converting Enzyme Inhibitor Peptide Sequence and Construction of Expression System[J].Journal of South China University of Technology(Natural Science Edition),2005,33(10):90-92. |
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| Authors: | Liu Dong Li Shi-min Zhang Li-jun Tang Yu-qian Liang Shi-zhong |
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| Institution: | 1. College of Biological Science and Engineering, South China Univ. of Tech., Guangzhou 510640, Guangdong, China; 2. School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, Guangdong, China |
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| Abstract: | Five angiotensin-converting enzyme inhibitor peptide (ACEIP) sequences were first designed and synthesized according to the relationship between the function and the structure of ACEIP. Next, the optimal peptide, namely, P3(six-amino-acid sequence) was determined by the bioactivity measurement and the digesting test in vitro. It was then jointed at the specific enzyme cleavage site to construct a six-copy fusion peptide. The corresponding coding gene was cloned into pGEX-4T-2. The fusion expression vector of pGEX-4T-2-ACEIP was finally transformed into Escherichia coli BL21. The results of the double enzyme-cleavage, PCR and the sequence analysis all indicate that the recombinant plasmid pGEX-4T-2-ACEIP is successfully constructed. |
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| Keywords: | angiotensin-converting enzyme inhibitor peptide sequence design gene recombination |
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