| 苯乙烯类菁染料pH探针的合成与活细胞成像 |
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| 引用本文： | 王炳帅,王丽,吴彤,杨志刚,彭孝军.苯乙烯类菁染料pH探针的合成与活细胞成像[J].高等学校化学学报,2010,31(6):1148-1151. |
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| 作者姓名： | 王炳帅 王丽 吴彤 杨志刚 彭孝军 |
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| 作者单位： | 大连理工大学精细化工国家重点实验室, 大连 116012 |
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| 摘 要： | 合成了一个以苯乙烯类菁染料为母体的含胺类基团的pH荧光探针, 合成方法简单, 产物收率高, 提纯方便. 在乙醇-水体系中的性能测试结果表明, 随着pH值的降低, 其非质子化形态的吸收峰强度逐渐降低, 而质子化形式的长波长吸收峰强度逐渐升高, 长、短波长相差(Δλ)130 nm, 颜色由黄色变成粉红色, 利于可视观察; 探针在氨基呈非质子化形式时没有荧光, 氨基质子化后探针的荧光强度显著增强. 活细胞实验表明, 探针可以穿透细胞膜, 在细胞质内pH值偏低区域显示红色荧光, 可以用来定性探测酸性细胞器.
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| 关 键 词： | 苯乙烯类菁染料 pH探针 活体细胞莹光成像 酸性细胞器 |
| 收稿时间： | 2009-09-25 |
Synthesis of pH Probe Based on Styrylcyanine Dyes and Fluorescence Image in Live Cell |
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| WANG Bing-Shuai,WANG Li,WU Tong,YANG Zhi-Gang,PENG Xiao-Jun.Synthesis of pH Probe Based on Styrylcyanine Dyes and Fluorescence Image in Live Cell[J].Chemical Research In Chinese Universities,2010,31(6):1148-1151. |
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| Authors: | WANG Bing-Shuai WANG Li WU Tong YANG Zhi-Gang PENG Xiao-Jun |
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| Institution: | State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China |
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| Abstract: | Fluorescent pH indicators have been widely applied in recent years to monitor changes in intracellular pH.Though some pH probes were synthesized and applied to sense the acidic organelles in live cells.However,the syntheses of these probes were very complicated.So,one pH fluorescence probe based on amino-styrylcyanine was synthesized with good yield and its purification was easy.Its abilities as pH probe were tested in ethanol-water solution.The results showed the absorption intensity of the base form(short... |
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| Keywords: | Styrylcyanine dye pH probe Live cell fluorescence image Acidic organelle |
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