| 傅立叶变换离子回旋共振质谱分析与鉴定骨桥蛋白的多肽片段 |
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| 引用本文： | 蒋鑫萍,程舸,于雷,王韶,刘晓梅,石磊. 傅立叶变换离子回旋共振质谱分析与鉴定骨桥蛋白的多肽片段[J]. 分析化学, 2009, 37(9) |
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| 作者姓名： | 蒋鑫萍 程舸 于雷 王韶 刘晓梅 石磊 |
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| 作者单位： | 吉林大学公共卫生学院,长春,130021 |
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| 基金项目： | 吉林省科技厅白求恩医学专项基金 |
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| 摘 要： | 采用高效液相色谱(HPLC)SCX及C4色谱柱从人乳样品的乳清部分分离与纯化出骨桥蛋白(Osteopontin, OPN),在一定条件下将其用胰蛋白酶酶解后,利用高分辨傅立叶变换离子回旋共振质谱(FT-ICR-MS),结合微喷雾(Nano-spray)技术,并借助Mascot软件数据库,获得了OPN相关多肽片段GDSVVYGLR和QNLLAPQTLPSK的质谱结构信息,分析了其裂解机理.研究表明,利用FT-ICR-MS并结合HPLC等化学方法,可以高效、快速地分离与纯化人乳样品中的OPN,借助Mascot软件数据库进行质谱分析,能够简洁、准确地鉴定相关多肽片段的结构信息,为OPN多肽片段不同翻译后修饰位点的确定与其生物学活性关系的研究提供了理论依据.
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| 关 键 词： | 傅立叶变换离子回旋共振质谱 人乳 骨桥蛋白 多肽片段 |
Accurate Analysis and Identification of Polypeptide Fragmentations of Osteopontin by Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry |
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| JIANG Xin-Ping,CHENG Ge,YU Lei,WANG Shao,LIU Xiao-Mei,SHI Lei. Accurate Analysis and Identification of Polypeptide Fragmentations of Osteopontin by Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2009, 37(9) |
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| Authors: | JIANG Xin-Ping CHENG Ge YU Lei WANG Shao LIU Xiao-Mei SHI Lei |
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| Affiliation: | JIANG Xin-Ping,CHENG Ge,YU Lei,WANG Shao,LIU Xiao-Mei,SHI Lei(College of Public Health,Jilin University,Changchun 130021) |
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| Abstract: | Fourier transform-ion cyclotron resonance-mass spectrometric(FT-ICR-MS) technique has the significant advantages in sensitivity and dynamic range for the detection of different fragmentations of peptides and proteins at lower levels.The osteopontin(OPN) was isolated and purified from the healthy human milk using HPLC by SCX and different types of C4 columns combined with FT-ICR-MS and Mascot software search.After digesting the purified OPN sample by the specific enzyme,some correlative mass structural infor... |
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| Keywords: | Fourier transform-ion cyclotron resonance-mass spectrometry human milk osteopontin polypeptide fragmentations |
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