微流控芯片快速鉴定黄山松DNA多态性片段

黄山松是中国山区特有的优质针叶松，有关其遗传图谱的建立一直受到人们的关注，然而迄今为止，运用分子标记法来建立其遗传图谱尚未见报道．随机引物扩增DNA多态性标记(简称RAPD)可快速提供联锁信息，尤其适用于建立针叶树类的基因图谱，通常采用平板凝胶电泳对多态性的

Fast Identification of Polymorphic RAPD Fragments of P.Taiwanensis Hayata Using an Integrated Microfluidic Chip-based System

The microfluidic chip-based system in conjunction with the t-test, was found applicable to identify the polymorphic RAPD fragments from P. Taiwanensis Hayata, whose polymorphism was weak and the sizes of each allele were unknown. The suggested microchip electrophoresis was very sensitive, and it requires much less sample and only a quarter of the time compared to the agarose gel method. The standard deviations of the fragment sizes and peaks area, for replicates number 10, ranged from 0 97%(701 bp) to 1%(301 bp) and 10%(301 bp) to 11 9%(701 bp), respectively. The average values of three alleles in the polymorphic RAPD fragments were identified as 301, 708 and 1069 bp with standard deviations of 3, 7, and 12 bp.