Photosensitized NADH formation system with multilayer TiO2 film |
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Authors: | Sagawa Takashi Sueyoshi Ryota Kawaguchi Mikako Kudo Mayu Ihara Hirotaka Ohkubo Katsutoshi |
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Affiliation: | Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan. t-sagawa@iae.kyoto-u.ac.jp |
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Abstract: | A TiO(2)/polymer film on a quartz plate fabricated by a layer-by-layer method was employed for NADH production from NAD(+) with lipoamide dehydrogenase and methyl viologen in Tris-HCl buffer on irradiation with UV light, and the ultra thin film prevented from enzyme deactivating effectively. |
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