涂敷型直链淀粉手性固定相直接拆分外消旋硫代缩水甘油醚

合成并制备了直键淀粉-三（苯基氨基甲酸酯）涂敷型手性固定相，利用高效波相色谱法首次直接拆分了4种外消旋硫代缩水甘油醚，并考察了流动相中异丙醇体积分数对拆分及保留的影响。

Chiral Separation of Thio-Glycidyl Ether on Amylose-Chiral Stationary Phase by High Performance Liquid Chromatography

In the present study, we synthesized amylose-tris (phenylcarbamate), and used it as chiral stationary phase based on silica gel. This stationary phase offered a practically useful chiral separation medium.Amylose was allowed to react with an excess of phenylisocyanate in pyridine for 48 h. The obtained carbamate was dissolved in DMF and adsorbed on silica gel previously treated with 3 -aminopropyltriethoxysilane.The mass ratio of the carbamate to the silica gel was 0. 45/2. 55. The four thio-glycidyl ethers were resolved on the amylose tris (phenylcarbamate) chiral stationary phase. Separation was carried out with a hexane / 2-propanol mixture as mobile phase at a flow rate of 1. 0 mL/min at room temperature. The effects of 2-propanol concentration on the retention and resolution have been investigated.