| 快速蛋白质液相色谱法分离鉴定脱氧寡核苷酸片段 |
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| 引用本文： | 唐惠炜,金由辛,朱德煦.快速蛋白质液相色谱法分离鉴定脱氧寡核苷酸片段[J].色谱,1999,17(5):438-440. |
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| 作者姓名： | 唐惠炜 金由辛 朱德煦 |
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| 作者单位： | [1]中国科学院上海生物化学研究所分子生物学国家重点实验室 [2]中国科学院上海生物化学研究所分子生物学国家重点实 |
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| 基金项目： | 国家自然科学基金，上海生命科学中心资助项目 |
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| 摘 要： | 由机器合成的反义核酸药物需要有效的纯度鉴定方法。用ＭＯＮＯ Ｑ柱在ｐＨ１２时以ＮａＣｌ的浓度梯度洗脱，可将１９至２１Ｎｔｓ的小片段寡核苷酸很好分开，因此快速蛋白质液相色谱法可用来分离鉴定反义核酸药物。
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| 关 键 词： | 反义核酸药物 寡聚脱氧核苷酸 快速蛋白质液相色谱法 |
Separation and Characterization of Oligodeoxynucleotide by Fast Protein Liquid Chromatography |
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| Tang Huiwei ,Jin Youxin ,Zhu Dexu\.Separation and Characterization of Oligodeoxynucleotide by Fast Protein Liquid Chromatography[J].Chinese Journal of Chromatography,1999,17(5):438-440. |
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| Authors: | Tang Huiwei Jin Youxin Zhu Dexu\ |
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| Institution: | State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China. |
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| Abstract: | The antisense drug synthesized by DNA synthesizer is an oligodeoxynucleotide (ODN). The purity of the drug should be determined effectively. Because the ODNs are molecules with negative charges at pH 12, they can be separated according to the quantity of charges with them on the ion\|exchange column. On anion exchange column, MONO\|Q, the ODNs ranged from 19 bases to 21 bases can be separated successfully by gradient elution of NaCl solution from 0 mol/L to 1.8 mol/L. The experiment proved that the fast protein liquid chromatography is a very useful tool for the determination of the antisense drug. |
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| Keywords: | fast protein liquid chromatography oligodeoxynucleotide antisense drug |
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