| 高效液相色谱法测定蜜蜂蜂体王浆酸 |
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| 引用本文： | 范晖,何承顺,韩红岩. 高效液相色谱法测定蜜蜂蜂体王浆酸[J]. 色谱, 1999, 17(3): 301-302 |
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| 作者姓名： | 范晖 何承顺 韩红岩 |
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| 作者单位： | 山东农业大学中心实验室!泰安，271018，山东农业大学中心实验室!泰安，271018，山东农业大学中心实验室!泰安，271018 |
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| 摘 要： | 建立了蜂体中10－HDA的高效液相色谱分析方法。对10－HDA的提取方法进行了研究。在选择的最佳色谱条件下，线性范围为10～1000ng，r=0．9998，回收率96．5％～99．2％，检测限为0.53μg／g。
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| 关 键 词： | 高效液相色谱法 蜜蜂蜂体 王浆酸 |
Determination of 10-HDA in Honeybee Body by HPLC |
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| Fan Hui, He Chengshun and Han Hongyan. Determination of 10-HDA in Honeybee Body by HPLC[J]. Chinese journal of chromatography, 1999, 17(3): 301-302 |
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| Authors: | Fan Hui He Chengshun Han Hongyan |
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| Affiliation: | Central Laboratory, Shandong Agricultural University, Taian, 271018. |
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| Abstract: | In the present work we found that in the honeybee body there exists an unsaturated fatty acid, trans-10-hydroxy-2-decenoic acid (10-HDA), which was known only to be present in royal jelly. We established the analytical method of 10-HDA in honeybee body by HPLC and simplified the extraction method of 10-HDA. In the optimum conditions the linear range of detection was 10-1,000 ng, the correlation coefficient was 0.9998, the recovery was 96.5%-99.2% and the detectable limit was 0.53 microgram/g. |
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| Keywords: | ?high performance liquid chromatography honeybee body 10-HDA |
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