高效液相色谱法测定低聚果糖的组分

采用高效液相色谱法（ＨＰＬＣ），以μ－ＢｏｎｄａｐａｋＣ１８柱为分析柱，水为流动相，示差折光仪为检测器，一次进样能同时测定低聚果糖样品中的果糖、蔗糖、三糖、四糖、五糖、六糖等。测定了“博爱生命素”样品，所得结果良好，变异系数为０．５５％～１．１％，线性相关系数为０．９９９２～１．０００。低聚果糖的最低检出限在微克级。

Analysis of the Composition in Fructooligosaccharide by High Performance Liquid Chromatography

In this paper, an analytical method for determination of fructooligosaccharide by high performance liquid chromatography(HPLC) is presented. The HPLC conditions are mu-Bondapak C18 column, 300 mm x 3.9 mm i.d., water as mobile phase and refractive index detector. Monosaccharide, disaccharide, trisaccharide, tetrasaccharide, pentasaccharide, hexasaccharide etc. can be simultaneously determined in a single injection. The method has been used to determine the fructooligosaccharides in "Boai biogen". The results are satisfactory. The CVs were 0.55%-1.1% and correlation coefficients were 0.9992-1.000. The lowest detectable limit for fructooligosaccharide was in microgram level.