| 柱前手性衍生化反相高效液相色谱法分离拉贝洛尔对映体 |
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| 引用本文： | 赵华,李惠芝,邱宗荫.柱前手性衍生化反相高效液相色谱法分离拉贝洛尔对映体[J].色谱,1999,17(4):369-371. |
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| 作者姓名： | 赵华 李惠芝 邱宗荫 |
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| 作者单位： | 重庆医科大学药物化学与生物材料研究室!重庆，400046，重庆医科大学药物化学与生物材料研究室!重庆，400046，重庆医科大学药物化学与生物材料研究室!重庆，400046 |
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| 摘 要： | 以乙酰葡萄糖异硫氰酸酯（GITC）作柱前手性衍生化试剂，用反相高效液相色谱法成功地分离了拉贝洛尔的两对对映异构体，并以荧光检测和紫外检测作对照，确认了4个衍生物的色谱峰。
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| 关 键 词： | 反相高效液相色谱法 光学异构体 拉贝洛尔 乙酰葡萄糖异硫氰酸酯（GITC） |
Separation of Enantiomeric Labetalol by Reversed-Phase High Performance Liquid Chromatography |
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| Zhao Hua, Li Huizhi and Qiu Zongyin.Separation of Enantiomeric Labetalol by Reversed-Phase High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,1999,17(4):369-371. |
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| Authors: | Zhao Hua Li Huizhi and Qiu Zongyin |
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| Institution: | Chongqing University of Medical Sciences, Chongqing, 400046. |
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| Abstract: | A reversed-phase high performance liquid chromatographic method for the separation of labetalol enantiomers was developed. In the method, 2, 3, 4, 6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (GITC) was chosen as the reagent for pre-column chiral derivatization of labetalol to give four diastereomeric thiourea derivatives. These derivatives were efficiently separated on a Nova-Pak C18 column using V(MeOH):V(0.01 mol.L-1(pH 7.00) H2PO4(-)-HPO(4)2- buffer) = 51:49 as the mobile phase and detected by UV detector at a wavelength of 250 nm or fluorescence detector at lambda ex = 340 nm and lambda em = 440 nm. The effects of the pH of mobile phase on the retention and fluorescence absorbance are also discussed. |
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| Keywords: | NULL |
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