蜂蜜中残留抗生素的填充毛细管液相色谱法检测

采用新型毛细管高效液相色谱法和自行填充的毛细管色谱柱，以四环素、土霉素和金霉素为标准物，对蜂蜜中残留的四环素类抗生素进行了定量分析。方法具有操作简单、灵敏度高、定量准确的优点。在10μg/L至0.4mg/L的质量浓度范围内，土霉素、四环素和金霉素的线性相关系数分别为rOTC=0.99695，rTC=0.99778和rCTC=0.98836。毛细管高效液相色谱法可用于抗生素类物质的高灵敏度测定。

Detection of Residual Antibiotics in Honey by Capillary High Performance Liquid Chromatography

The quantitative analysis of the three antibiotics-tetracycline(TC), oxytetracycline(OTC) and chlortetracyclinum(CTC) was carried out with a self-packed Hypersil C18 150 mm x 0.5 mm i.d. column. As little as 0.5 nanogram of antibiotics can be well detected, the capillary high performance liquid chromatographic method is 100 times more sensitive than that with ordinary HPLC method. The coefficients of linear correlation of mass concentration from 0.5 nanogram to 20 nanogram were rOTC = 0.99695, rTC = 0.99778 and rOTC = 0.98836. It showed that capillary HPLC is a good method used for sensitive analysis of antibiotics in honey.