微等电聚焦分离等位基因表达蛋白的研究

借助于显微操作方法，用等电聚焦和改进的超敏感染色技术对西葫芦单个花粉粒中的可溶性蛋白进行了电泳分析，获得了清晰的电泳图谱。在靠近酸性端有两条相距极近而且分布比较特殊的蛋白带，在第１种植株中，这两条带稍微靠近酸性端；在第２种植株中，它们稍微远离酸性端；在第３种植株中，它们相同于第１种和第２种，而且在两种之间的分布比例为１∶１。就这两条带的分布情况在判断植株的相对纯合性或杂合性方面的应用以及单花粉蛋白等电聚焦技术配合显微操作方法在群体遗传学和发育生物学研究方面的应用作了讨论。

Study on Proteins of Allele by Using the Microisoelectric Focusing

Clear protein electrophoresis pattern of single pollen grain from Cucurbita pepo L. was obtained with isoelectric focusing and supersensitive stain technique. The results showed that there were two special distributing protein bands near acidity pole. In species I, the two protein bands were a little near to acidity pole and in species II, that was a little far from acidity pole. In species III, the two protein bands distributed either like species I or like species II and the distributed ratio was 1:1. Moreover, we discuss how to identify relative purity of gene with the sorts of the special proteins.