用时间分辨光谱研究红细胞悬液的荧光发射

实验获得了激光照射红细胞悬液的荧光光谱,并分别监测不同荧光峰值波长处强度随时间的衰变过程,测试了其相应的荧光寿命。结果表明,在波长为407nm的激光照射下,红细胞悬液向外发射中心波长分别位于596,628,692nm的荧光光谱,各荧光峰对应衰变过程的平均荧光寿命分别为1.97,13.31,14.58ns。利用荧光强度和吸收率的加和性表示了混合物的总吸收率和总荧光发射强度,通过理论计算获得了红细胞悬液中锌卟啉、原卟啉和其他游离物参与荧光发射的相对含量和相对强度在不同荧光峰位的变化关系,进一步解释了不同峰位处荧光发射强度和平均荧光寿命的变化原因。

Fluorescent Emission of Erythrocyte Suspension with Time-Resolved Fluorescence Spectrometer

The fluorescence spectrum of erythrocyte suspension irradiated by laser is studied experimentally. The decay processes of fluorescence intensity are monitored at the emission band with different fluorescence peaks. The results show that when erythrocyte suspension is irradiated by laser with wavelength of 407 nm, there are three fluorescence peaks locate at 596, 628 and 692 nm, respectively. The average fluorescence lifetimes of each decay process in turn are 1.97, 13.31 and 14.58 ns. The total absorptivity and total fluorescence counts are expressed based on the theory of additive property of absorptivity and fluorescence intensity. By theoretical analysis, the variations of relative concentration and relative fluorescence intensity at different peak have been obtained in erythrocyte suspension, which are protoporphyrin, zinc protoporphyrin and other free substances. The change of fluorescence intensity and average lifetime in different fluorescence peaks are illuminated.