Improving selenium extraction by sequential enzymatic processes for Se-speciation of selenium-enriched Agaricus bisporus |
| |
Authors: | M Dernovics Zs Stefánka P Fodor |
| |
Institution: | Department of Applied Chemistry, Faculty of Food Science, Szent István University, Budapest, Viilányt út, Hungary. dernovics@omega.kee.hu |
| |
Abstract: | Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 microg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes--Lysing enzyme and Driselase--in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestion. A 3-step procedure applying Lysing enzyme and pronase gave the highest extraction efficiency (89%); however, the best species recovery was achieved by a one-step proteolytic procedure. All the procedures of selenium speciation were controlled by independent ICP-AES analysis measuring the total amount of selenium. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |