Validation and Comparative Studies of Four Sulfonamide Immunoassays Based on the Same Generic Polyclonal Antibody |
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| Authors: | Hongyan Zhang Yan Zhang Shuo Wang Liguo Zang |
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| Institution: | (1) Key Laboratory of Food Nutrition and Safety, Ministry of Education, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300222, People’s Republic of China;(2) College of Life Science, Shandong Normal University, Jinan, 250014, People’s Republic of China; |
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| Abstract: | Four different immunoassays based on the same generic polyclonal antibody were validated by high performance liquid chromatography,
respectively. They also were compared with each other in terms of sensitivity, precision, and accuracy for the quantification
or screening of sulfonamide residues in food samples. Correlation studies showed that there was a good correlation between
the immunoassays and liquid chromatography data. The conventional plate assay has better precision and the plate-enhanced
chemiluminescent assay has higher sensitivity. These two methods all could be used as quantification methods for large numbers
of samples and complements of the conventional analytical methods in laboratory. The flow-through strip assay has higher sensitivity
and the dip-stick strip assay was less affected by matrix effect. These two methods all could be used as valuable tools for
the rapid on-site screening of sulfonamide residues in animal-derived food samples. |
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