Centrifugal separation in the recovery of intracellular protein from E. coli |
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| Institution: | 1. Department of Urology, All India Institute of Medical Sciences, New Delhi, India;2. Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India;1. Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, China;2. Department of Physiology, Nanjing University of Chinese Medicine Hanlin College, Taizhou, China;3. Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou, China;4. Department of Pathology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China |
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| Abstract: | A 1000 l fermenter has been used to produce a feed of E. coli containing high levels of β-galactosidase. The individual unit operations have been investigated for the primary recovery of the enzyme, i.e. cell harvesting, cell disruption and cell debris removal. The cell separation yield was found to be in excess of 99%. Four passes through a high-pressure homogenizer released 95% of the enzyme. Approximately 87% of the β-galactosidase could be recovered after cell debris removal. The performance of the centrifuge during solid-liquid separation could be modelled using simple curve-fitting techniques. |
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