高效液相色谱二极管阵列检测法同时测定丹参滴注液中四种水溶性成分的含量

建立了高效液相色谱二极管阵列检测(HPLC-DAD)法同时测定丹参滴注液中丹参素、原儿茶醛、迷迭香酸和丹酚酸B四种水溶性成分的含量。采用DiamonsilTMC18色谱柱(250×4.6 mm,5μm),以甲醇和5%冰乙酸为流动相进行梯度洗脱,流速为1.0mL/min,柱温30℃,检测波长为286 nm。在此色谱条件下四种水溶性成分可完全分离。丹参素、原儿茶醛、迷迭香酸和丹酚酸B的线性范围分别为0.2192~1.934μg(r=0.9999),0.03508~0.2456μg(r=1.0000),0.2592~1.814μg(r=1.0000),0.3864~2.705μg(r=0.9999)。平均回收率丹参素为102.6%,相对标准偏差(RSD)为0.55%;原儿茶醛为103.5%,RSD为0.42%;迷迭香酸为99.8%,RSD为0.68%;丹酚酸B为102.8%,RSD为0.49%。该方法简单、快速,四组分分离良好,可用于丹参滴注液的质量控制。

Simultaneous Determination of 4 Water-soluble Components in Danshen Dripping Solution by High Performance Liquid Chromatography with Diode Array Detection

A high performance liquid chromatographic method with diode array detection was established for simultaneous determination of 4 water-soluble compounds of danshensu, protocatechuic aldehyde,rosmarinic acid and salvianolic aicd B in danshen dripping solution.The column used was Diamonsil~(TM)C_(18)(250×4.6 mm,5 μm). The mobile phase was methanol and 5% glacial acetic acid with gradient elution and the flow rate was 1.0ml/min, the column temperature was 30℃, and the detection wavelength was 286 nm. The linearity was obtained over the range of 0.2192～1.934 μg(r=0.9999)for danshensu with average recovery of 102.6%(RSD=0.55%), 0.03508～0.2456 μg(r=1.0000)for protocatechuic aldehyde with average recovery of 103.5%(RSD=0.42%),0.2592～1.814 μg(r=1.0000)for rosmarinic acid with average recovery of 99.8%(RSD=0.68%), 0.3864～2.705 μg(r=0.9999)for salvianolic aicd B with average recovery of 102.8%(RSD=0.49%), respectively. The method is rapid, accurate and reliable, and can be used to control the quality of Danshen dripping solution.