铽荧光纳米配合物的制备及其潜血手印显现应用

以铽离子为发光中心、对苯二甲酸为第一配体、菲咯啉为第二配体，采用化学方法一步制备出表面羧基修饰的铽荧光纳米配合物。使用活化剂1-（3-二甲氨基丙基）-3-乙基碳二亚胺盐酸盐配合稳定剂N-羟基琥珀酰亚胺对配合物表面的羧基进行活化，促使活化羧基与潜血手印中的胺基在温和条件下迅速发生酰胺反应，成功实现了潜血手印的靶向显现。优化了潜血手印显现的最佳条件，显现悬浮液中配合物与水的质量比推荐为1：35，显现浸泡时间推荐为20 s。并深入探究了手印显现的对比度、灵敏度、选择性、适用性。实验结果表明，制备的表面活化羧基修饰的铽荧光纳米配合物适用于光滑非渗透性及半渗透性客体表面潜血手印的高质量与高效率显现。

Synthesis of terbium fluorescent nanocomplexes and their applications in the development of latent bloody fingerprints

Carboxyl functionalized terbium fluorescent nanocomplex was chemically synthesized by a one-step process using terbium ion as the luminescence center, p-phthalic acid as the first ligand, and phenanthroline as the second ligand. The carboxyl groups on the surface of these nanocomplexes were further activated using 1-(3-dimethyl-amino-propyl)-3-ethylcarbodiimine hydrochloride as the activator combined with N-hydroxy succinimide as the stabilizer. Due to the rapid and mild amide reaction between the activated carboxyl groups on the surface of the nano-complexes and the amino groups in bloody fingerprint residuals, the resulting nanocomplexes were finally used as fluorescent probes for the targeted development of latent bloody fingerprints. Excited with 254 nm ultraviolet light, the papillary ridges could emit strong green fluorescence, which could give a sufficient contrast between the developing signal and the background noise; the papillary ridges were coherent, the minutiae were sharp, and the sweat pores were distinct; the contrast between the ridges and the furrows were obvious. The parameters for fingerprint development were optimized, namely, the mass ratio of nanocomplex to water in suspension was recommended to be 1:35, and the period for staining was recommended to be 20 s. In addition, the contrast, sensitivity, selectivity, and applicability in fingerprint development were also investigated in detail. Experimental results show that the activated carboxyl functionalized terbium fluorescent nanocomplexes are suitable for developing the latent bloody fingerprints on smooth non- and semi-porous surfaces with high quality and high efficiency.