Lactic acid production through cell-recycle repeated-batch bioreactor |
| |
Authors: | Hurok Oh Young-Jung Wee Jong-Sun Yun Hwa-Won Ryu |
| |
Institution: | (1) Faculty of Applied Chemical Engineering, Institute of Bioindustrial Technology, Chonnam National University, 500-757 Gwangju, Korea |
| |
Abstract: | The effect of various nitrogen sources on cell growth and lactic acid production was investigated. The most effective nitrogen
source was yeast extract; more yeast extract gave higher cell growth and lactic acid productivity. Yeast extract dosage and
cell growth were proportional up to a yeast extract concentration of 30 g/L, and lactic acid productivity was linearly correlated
up to a yeast extract dosage of 25 g/L. However, increasing the yeast extract content raises the total production cost of
lactic acid. Therefore, we attempted to find the optimum yeast extract dosage for a repeated-batch operation with cell recycling.
The results show that when using Enterococcus faecalis RKY1 only 26% of the yeast extract dosage for a conventional batch fermentation was sufficient to produce the same amount
of lactic acid, whereas the lactic acid concentration in the product stream (92–94 g/L) and lactic acid productivity (6.03–6.20
g/L·h]) were similar to those of a batch operation. Furthermore, long-term stability was established. |
| |
Keywords: | Lactic acid repeated-batch cell-recycle hollow-fiber module nitrogen source yeast extract |
本文献已被 SpringerLink 等数据库收录! |
|