High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection |
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Authors: | Kne?evi? Ana Bones Jonathan Kra?un Stjepan Kre?imir Gornik Olga Rudd Pauline M Lauc Gordan |
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Institution: | Genos Ltd, Laboratory for Glycobiology, Zagreb, Croatia. |
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Abstract: | A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day. |
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