Application of optimization procedures for the separation of anabolic compounds by high-performance liquid chromatography |
| |
| Authors: | E H Jansen R Both-Miedema R H van den Berg |
| |
| Institution: | Laboratory for Clinical Chemistry, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands. |
| |
| Abstract: | A simple systematic approach is presented for optimizing high-performance liquid chromatographic separations of anabolics with multi-component isocratic mobile phases. A computer program was obtained and adjusted for use with an IBM-compatible XT personal computer. The program requires experimental retention data with three quaternary solvent mixtures to calculate the optimum solvent composition using a geometric model of a prism. For each possible composition of the mobile phase the set of retention data can be calculated. Applications are shown for mixtures of anabolic compounds using a mobile phase composed of methanol, tetrahydrofuran and acetonitrile. The predicted retention data agreed very well with the experimental data. |
| |
| Keywords: | |
|
|
