Label-free detection of amino acids using gold nanoparticles in electrokinetic chromatography-thermal lens microscopy |
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Authors: | Kitagawa Fumihiko Akimoto Yoshihiro Otsuka Koji |
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Institution: | Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. |
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Abstract: | To achieve label-free detection of amino acids in capillary-based electrokinetic chromatography-thermal lens microscopy (EKC-TLM), gold nanoparticles (GNPs), which possess the absorption around 500nm attributed to surface plasmon resonance (SPR), were added to the background solution (BGS). Since the SPR absorption of the GNPs exhibits a sensitive response toward environmental changes and the degree of aggregation, the sensitive detection of non-absorbing species is expected by using the GNPs in EKC-TLM (GNP-EKC-TLM). In the GNP-EKC-TLM analysis of glutamic acid (Glu), a sharp peak was observed when the GNPs were added to the BGS. The plot of the peak area of Glu against its concentration gave a good linear relationship and the limit of detection was estimated to be 25mug/mL. Furthermore, a baseline separation of lysine and Glu was successfully achieved. Thus, the EKC separation and label-free TLM detection of the amino acids can be realized only by adding the GNPs into the BGSs. |
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Keywords: | Electrokinetic chromatography Thermal lens microscope Gold nanoparticles Label-free detection Amino acids |
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