A comparative study of large volume injection techniques for microbore columns in HPLC |
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Authors: | H A Claessens M A J Kuyken |
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Institution: | (1) Laboratory of Instrumental Analysis, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands;(2) Central Laboratory Elcoma, N. V. Philips, P.O. Box 218, 5600 MD Eindhoven, The Netherlands |
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Abstract: | Summary This paper focuses attention on the potentially larger signal-to-noise ratios produced by microbore columns in comparison
with conventional columns. The increased chromatographic signals by the application of microbore columns are due to the lower
chromatographic dilution of elution profiles which are proportional to the square of the column inner radius. Generally less
than 1μl sample should be injected into microbore systems to obtain the full benefit of the column performance. However, since
more sample can be loaded on conventional columns compared to microbore columns the advantage of improved signal-to-noise
ratio can only be realised in situations where very little sample is available. To inject more than 1μl sample, at the same
time avoiding extra band-broadening effects, suitable injection techniques must be available.
In this study three injection methods for microbore systems that meet this condition, are studied and compared. |
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Keywords: | Column liquid chromatography Microbore columns Injection techniques |
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