基质固相分散液相色谱法检测辣椒产品中的黄曲霉毒素

建立了中性氧化铝-石墨化碳黑的基质固相分散共柱提取净化前处理和以溴为衍生试剂的液相色谱-柱后在线衍生荧光检测法,并将该方法用于辣椒产品中黄曲霉毒素B1,B2,G1,G2的分析。对固相分散剂及共柱净化剂进行了选择和优化。该方法对B1,B2,G1,G2的平均回收率分别为95.4%,87.3%,91.5%和92.6%;方法对B1,G1的检出限为0.25 ng/g,对B2,G2的检出限为0.10 ng/g;对B1,B2,G1,G2进行测定的相对标准偏差(RSD)分别为3.3%,5.8%,4.7%和6.1%。对基质固相分散法和免疫亲和柱法的净化效果进行了比较,结果表明基质固相分散提取净化可以作为一种有效的方法用于辣椒产品中黄曲霉毒素的测定。

Determination of Aflatoxins in Hot Chilli Products by Matrix Solid-Phase Dispersion and Liquid Chromatography

A new method based on matrix solid-phase dispersion(MSPD) extraction with neutral alumina and co-column purification with graphitized carbon black has been developed to determine aflatoxins B1,B2,G1,G2 in hot chilli products.The method includes liquid chromatography and fluorescence detection with on-line post-column derivatization with bromine.Optimization of different parameters,such as the type of solid supports for matrix dispersion and co-column clean-up was carried out.The recoveries of aflatoxins B1,B2,G1 and G2 were 95.4%,87.3%,91.5% and 92.6%,respectively,with relative standard deviations ranging from 3.3% to 6.1%.The limits of detection were in the range of 0.10 ng/g(B2,G2) to 0.25 ng/g(B1,G1).In addition,the comparison of the extraction and purification effect of MSPD with immunity affinity column showed that,MSPD is a valid method to analyze aflatoxins in hot(chilli) products.