A Carbon Paste Electrode Modified by Bentonite and l‐Cysteine for Simultaneous Determination of Ascorbic and Uric Acids: Application in Biological Fluids |
| |
| Authors: | Prof. Dr. Mohamed Choukairi,Prof. Dr. Dounia Bouchta,Prof. Dr. Loubna Bounab,Prof. Dr. Elisa Gonzá lez‐ Romero,Prof. Dr. Mohamed Achache,Prof. Dr. Khalid Draoui,Prof. Dr. Faiza Chaouket,Prof. Dr. Ihssane Raissouni,Dr. Mouad Gharous |
| |
| Affiliation: | 1. Laboratory of Materials and Interfacial Systems, Faculty of Science, Abdelmalek Essaadi University, B.P. 2121, 93002 Tetouan Morocco ; 2. Laboratory of Information System and Software Engineering, National School of Applied Sciences of Tetouan, Abdelmalek Essaadi University, 93000 Tetouan Morocco ; 3. Department of Analytical Chemistry and Food, University of Vigo-Campus, 36310 Vigo Spain |
| |
| Abstract: | A novel modification of a paste carbon electrode by Bentonite (Bent) and l‐Cysteine (l‐Cyst) was carried out for uric acid (UA) and ascorbic acid (AA) detection and quantification. Morphological and compositional characterization of the electrode surface were carried out using electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy dispersive X‐ray spectroscopic analysis (EDS). Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were used to analyze UA and AA. The obtained sensor shows a good stability, sensibility, selectivity, and regeneration ability. Accordingly, the limit of detection (LOD) is found to be 0.031 μm and 9.6 μm for UA and AA, respectively. A good linearity in the range of 0.1 to 100 μm for UA and 10 to 1000 μm for AA was obtained. The peak‐to‐peak separation of UA‐AA (ΔE UA‐AA) was determined to be 330 mV. In addition, the sensor is applied successfully to monitor UA and AA in serum samples. |
| |
| Keywords: | ascorbic acid bentonite carbon paste electrode l-cysteine uric acid |
|
|
