| 二氢杨梅素-锌配合物的合成及其与DNA相互作用研究 |
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| 引用本文： | 袁娟,郭清泉,曾金华,舒绪刚. 二氢杨梅素-锌配合物的合成及其与DNA相互作用研究[J]. 分析测试学报, 2012, 31(8): 982-986 |
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| 作者姓名： | 袁娟 郭清泉 曾金华 舒绪刚 |
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| 作者单位： | 1. 广东工业大学轻工化工学院,广东广州,510006
2. 仲恺农业工程学院化学化工学院,广东广州,510225 |
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| 基金项目： | 广东省科技计划资助项目(2010B030700072) |
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| 摘 要： | 合成了二氢杨梅素-锌配合物(DMY-Zn),采用紫外可见光谱、红外光谱、元素分析及热重分析(TG-DTA)法对其结构进行了表征,结果表明二氢杨梅素与Zn2+离子形成了配合物,其组成为[C15H10O8Zn].2H2O。采用EB为荧光探针利用荧光滴定法和粘度法进一步研究了二氢杨梅素-锌配合物与DNA的相互作用,发现二氢杨梅素-锌配合物与DNA有较强的相互作用,能和EB竞争与DNA结合,插入到ctDNA相邻的碱基对中,其作用方式为插入作用,Stern-Volmer线性猝灭常数Ksq为1.01。
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| 关 键 词： | 二氢杨梅素-锌配合物 DNA 荧光光谱法 粘度法 相互作用 |
Study on Synthesis of Dihydromyricetin-Zinc Complex and Its Interaction with DNA |
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| YUAN Juan , GUO Qing-quan , ZENG Jin-hua , SHU Xu-gang. Study on Synthesis of Dihydromyricetin-Zinc Complex and Its Interaction with DNA[J]. Journal of Instrumental Analysis, 2012, 31(8): 982-986 |
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| Authors: | YUAN Juan GUO Qing-quan ZENG Jin-hua SHU Xu-gang |
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| Affiliation: | 1.School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,China; 2.Chemistry and Chemical Engineering,College of Zhongkai Agricultural Engineering,Guangzhou 510225,China) |
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| Abstract: | A complex of dihydromyricetin-Zn(DMY-Zn)was prepared firstly,the interaction of DMY-Zn complex with DNA was investigated by using fluorescence titration that ethidium bromide(EB) as a fluorescence probe and viscosity measurements.The results indicated that DMY and Zn2+ ions are easy to form complexes which compositions were tested as [C15H10O8Zn]·2H2O by characterization using UV-Vis,IR spectroscopy,elemental analysis and thermal gravimetric analysis(TG-DTA).With the strong interaction with DNA,the DMY-Zn complex can compete with the strong intercalator ethidium bromide to intercalate into the stacked base pairs through the intercalation which the Stern-Volmer quenching constant Ksq is equal to 1.01. |
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| Keywords: | dihydromyricetin-zinc complex DNA fluorescence spectrometry viscosity measurements interaction |
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