Evaluation of Rod‐Shaped Nanoparticles as Carriers for Gene Delivery |
| |
| Authors: | Cong Cheng Yan Liu Bang‐Jing Li Xun Sun Sheng Zhang |
| |
| Institution: | 1. State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute, Sichuan University, , Chengdu, 610065 PR China;2. Chengdu Institute of Biology, Chinese Academy of Sciences, , Chengdu, 610041 PR China;3. West China School of Pharmacy, Sichuan University, , Chengdu, 610041 PR China |
| |
| Abstract: | Fabrication of nonspherical particles for gene delivery remains a major challenge. In this study, novel rod‐like nanoparticles are prepared for efficient gene delivery by self‐assembly of α‐cyclodextrin (α‐CD) and polyethylenimine‐methoxy poly(ethylene glycol) (PEI‐mPEG). The study reveals that the rod‐like PEI‐mPEG/α‐CD particles can bind DNA effectively and the resulting PEI‐mPEG/α‐CD/DNA complexes show over four times higher gene delivery capability than their spherical counterparts and PEI(25K) due to more efficient cellular uptake. Furthermore, the cytotoxicity of rod‐like PEI‐mPEG/α‐CD is about five times lower than that of the nanospheres, and 50 times lower than that of DNA/PEI(25K). These results indicate that shape is an important parameter for the design of gene delivery vectors. |
| |
| Keywords: | self‐assembly cyclodextrin rod‐like nanoparticle gene delivery |
|
|
