首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Selective solid-phase extraction of amoxicillin and cephalexin from urine samples using a molecularly imprinted polymer
Authors:Beltran Antoni  Marcé Rosa M  Cormack Peter A G  Sherrington David C  Borrull Francesc
Institution:Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Abstract:In this paper we describe, for the first time, a molecularly imprinted polymer (MIP) for the antibiotic amoxicillin (AMX), synthesised by a noncovalent molecular imprinting approach and used to extract AMX selectively from urine samples. The MIP was applied as a molecularly selective sorbent in molecularly imprinted SPE (MISPE) in an off-line mode, where it showed useful cross-selectivity for a structurally related antibiotic, cephalexin (CPX). By using a MISPE protocol, the MIP was able to selectively extract both AMX and CFX from 5 mL of water spiked with 10 mg/L with recoveries of 75 and 78% for AMX and CFX, respectively. When applied to real samples (urine) at clinically relevant concentrations, recoveries from 2 mL of human urine spiked with 20 mg/L decreased slightly to 65 and 63% for AMX and CFX, respectively. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available SPE cartridges was performed. Improvements in the retention of both AMX and CFX on the MIP were obtained relative to the commercially available cartridges, and the MISPE extracts were considerably cleaner, due to molecularly selective analyte binding by the MIP.
Keywords:Amoxicillin  Cephalexin  Molecularly imprinted polymer  Solid‐phase extraction  Urine samples
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号