ICP-AES法测定粳稻近等基因系群体间糙米的矿质元素

采用电感耦合等离子体原子发射光谱法(ICP-AES)对粳稻近等基因系(NILs)BC₅F₅群体264个株系17种矿质元素(S, Mo, Ni, Fe, Cr, Na, Al, Cu, P, Sn, Zn, B, Mn, Mg, Ca, Sr, K)进行了测定。加标回收率为92.3%～110.2%, RSD<6%。结果表明: 以丽粳2号作供体及十和田受体培育出糙米多种高矿质元素基因定位的NILs，是迄今首次培育出粳稻孕穗期耐冷性及其关联矿质元素的近等基因系；该群体糙米中P, K, Mg, S, Ca, Zn, Mn, Na, Fe, Cu和Al等11种人体必须的生命元素含量较高; 群体间糙米矿质元素中五种宏量元素含量变异小(11.15%～16.45%)和12种微量元素变异大(16.57%～94.96%); 群体糙米17种矿质元素间136对性状间有95对性状呈显著或极显著相关，而与功能稻米活性成分有关的Mo，Ni，Al，Sn，Cr和Sr矿质元素15对性状相关(0.531**～0.921**)明显高于P，K，Ca，Mg和S宏量元素10对性状相关(0.175**～0.867**)。研究结果为糙米矿质元素的基因定位克隆提供了有益的参考数据。

Determination of Mineral Elements in Brown Rice of Near-Isogenic Lines Population for Japonica Rice by ICP-AES

In the present paper,the contents of 17 mineral elements(S,Mo,Ni,Fe,Cr,Na,Al,Cu,P,Sn,Zn,B,Mn,Mg,Ca,Sr and K) in brown rice of 264 plant lines in near-isogenic lines(NILs) population and parents for Japonica rice were determined by ICP-AES technique.The method proved to be simple,rapid,highly sensitive and accurate,and can be used to determine many elements at the same time.Its recovery ratio obtained by standard addition method ranged between 92.3% and 110.27%,and its RSD was lower than 6%. The analytical results showed that NILs for various mineral elements based on the BC5F5 population consisting of 261 lines were developed from a cross between NIL(BC4F5) and Towada.To our knowledge,this is the first report on that near-isogenic lines NIL(BC4F5) with the important value for high mineral elements associated with cold tolerance at booting stage have been bred.The results of the determination show that the brown rice is rich in eleven elements such as P,K,Mg,S,Ca,Zn,Mn,Na,Fe,Cu and Al necessary to human health.There is smaller variation of 5 macroelements(1 1.15%-16.45%) but larger variation of 12 microelements(16.57%-94.96%) in brown rice from populations.There is significant correlation among 95 from 136 pairs in 17 elements,moreover,especially the correlation(0.531 -0.921 ) among 15 pairs of six micro-elements(Mo,Ni,Al,Sn,Cr and Sr) associated with the active components of functional rice is relatively higher than that of 10 pairs(0.175 -0.867 ) of 5 macroelements(P,K,Ca,Mg and S),revealing the importance of microelements to catalysing and synthesizing the active components of functional rice.The above results provided reliable data and theory bases for gene location and cloning of controlling elements contents in brown rice,and further revealed the molecular and physiological mechanism of the relation between mineral elements in brown rice and cold tolerance at booting stage in japonica rice from Yunnan Province of China.