| 一种基于DPA识别基团的可视化检测锌离子的荧光增强型探针 |
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| 引用本文： | 王维娜,郑元梅,陈雪梅. 一种基于DPA识别基团的可视化检测锌离子的荧光增强型探针[J]. 无机化学学报, 2014, 30(4): 872-878 |
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| 作者姓名： | 王维娜 郑元梅 陈雪梅 |
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| 作者单位： | 湖北理工学院高等职业技术学院, 黄石 435003;湖北理工学院高等职业技术学院, 黄石 435003;湖北理工学院化学与材料工程学院, 黄石 435003 |
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| 基金项目： | 国家自然科学基金资助项目(No.51173060)。 |
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| 摘 要： | 以N,N-二(2-吡啶甲基)胺(DPA)为识别基团,设计合成了一种用于检测Zn2+的荧光增强型探针WN,在CH3CH2OH/TrisHCl(1∶9,V/V,pH=7.4)缓冲溶液中研究了它对Zn2+的识别特性。实验结果表明,WN对Zn2+有较高的选择性和灵敏度,它们之间的结合比为1∶1,对Zn2+的检出限为1.14×10-8 mol·L-1。WN能够快速地可视化检测Zn2+,在HeLa活细胞中对Zn2+的荧光显微成像表明WN可应用于生物体的检测。
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| 关 键 词： | DPA 锌离子 荧光探针 可视化检测 荧光成像 |
| 收稿时间： | 2013-10-13 |
| 修稿时间： | 2013-12-09 |
A Fluorescence Enhancement Probe for Visual Detection of Zn2+ with Receptor Based on DPA |
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| WANG Wei-N,ZHENG Yuan-Mei and CHEN Xue-Mei. A Fluorescence Enhancement Probe for Visual Detection of Zn2+ with Receptor Based on DPA[J]. Chinese Journal of Inorganic Chemistry, 2014, 30(4): 872-878 |
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| Authors: | WANG Wei-N ZHENG Yuan-Mei CHEN Xue-Mei |
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| Affiliation: | Higher Vocational Technical School, Hubei Polytechnic University, Huangshi, Hubei 435003, China;Higher Vocational Technical School, Hubei Polytechnic University, Huangshi, Hubei 435003, China;School of Chemical and Materials Engineering, Hubei Polytechnic University, Huangshi, Hubei 435003, China |
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| Abstract: | Afluorescence enhancement probe WNfor detection of Zn2+ with receptor based on di-2-picolylamine (DPA) was synthesized and structurally characterized. Its recognition properties with Zn2+ had been investigated in CH3CH2OH/Tris-HCl buffer solution (1:9, V/V, pH=7.4). The results showed that WN exhibited good sensitivity and selectivity to Zn2+, and the stoichiometry of WN-Zn2+ complex was estimated to be 1:1, the detection limit of Zn2+ was estimated to be 1.14×10-8 mol·L-1. WN was used for fast visual detection of Zn2+ and its intracelluar Zn2+ imaging in living HeLa cells, indicated that it could be applied in biological detection. |
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| Keywords: | DPA zinc ion fluorescent probe visual detection fluorescent imaging |
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