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Design and concise synthesis of a novel type of green fluorescent protein chromophore analogue |
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| Authors: | Masahiro Ikejiri Moe Tsuchino Yoshiko Chihara Takao Yamaguchi Takeshi Imanishi Satoshi Obika Kazuyuki Miyashita |
| Affiliation: | Faculty of Pharmacy, Osaka Ohtani University , Nishikiori-Kita 3-11-1, Tondabayashi, Osaka 584-8540, Japan, and Graduate School of Pharmaceutical Sciences, Osaka University , Yamadaoka 1-6, Suita, Osaka 565-0871, Japan. |
| Abstract: | A small molecular model compound for the green fluorescent protein chromophore was readily synthesized by a novel condensation reaction of (thio)imidate with imino-ester via an aziridine intermediate. This compound showed fluorescence in the solid and frozen solution states but not in the solution state. Its fluorescent property was successfully applied in the detection of dsDNA. |
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