Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity |
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Authors: | Liyuan Zhang Jin Xu Liangliang Sun Junfeng Ma Kaiguang Yang Zhen Liang Lihua Zhang Yukui Zhang |
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Affiliation: | (1) Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China;(2) Graduate School of Chinese Academy of Sciences, Beijing, 100039, China;(3) Department of Hepatic-Biliary-Pancreas and Transplantation Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, China; |
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Abstract: | In this study, zirconium oxide (ZrO2) aerogel was synthesized via a green sol–gel approach, with zirconium oxychloride, instead of the commonly used alkoxide with high toxicity, as the precursor. With such material, phosphopeptides from the digests of 4 pmol of β-casein with the coexistence of 100 times (mol ratio) BSA could be selectively captured, and identified by MALDI-TOF MS. Due to the large surface area (416.0 m2 g−1) and the mesoporous structure (the average pore size of 10.2 nm) of ZrO2 aerogel, a 20-fold higher loading capacity for phosphopeptide, YKVPQLEIVPN[pS]AEER (MW 1952.12), was obtained compared to that of commercial ZrO2 microspheres (341.5 vs. 17.87 mg g−1). The metal oxide aerogel was further applied in the enrichment of phosphopeptides from 100 ng nonfat milk, and 17 phosphopeptides were positively identified, with a 1.5-fold improvement in phosphopeptide detection compared with previously reported results. These results demonstrate that ZrO2 aerogel can be a powerful enrichment material for phosphoproteome study. |
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