Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity |
| |
Authors: | Liyuan Zhang Jin Xu Liangliang Sun Junfeng Ma Kaiguang Yang Zhen Liang Lihua Zhang Yukui Zhang |
| |
Institution: | (1) Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China;(2) Graduate School of Chinese Academy of Sciences, Beijing, 100039, China;(3) Department of Hepatic-Biliary-Pancreas and Transplantation Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, China; |
| |
Abstract: | In this study, zirconium oxide (ZrO2) aerogel was synthesized via a green sol–gel approach, with zirconium oxychloride, instead of the commonly used alkoxide
with high toxicity, as the precursor. With such material, phosphopeptides from the digests of 4 pmol of β-casein with the
coexistence of 100 times (mol ratio) BSA could be selectively captured, and identified by MALDI-TOF MS. Due to the large surface
area (416.0 m2 g−1) and the mesoporous structure (the average pore size of 10.2 nm) of ZrO2 aerogel, a 20-fold higher loading capacity for phosphopeptide, YKVPQLEIVPNpS]AEER (MW 1952.12), was obtained compared to
that of commercial ZrO2 microspheres (341.5 vs. 17.87 mg g−1). The metal oxide aerogel was further applied in the enrichment of phosphopeptides from 100 ng nonfat milk, and 17 phosphopeptides
were positively identified, with a 1.5-fold improvement in phosphopeptide detection compared with previously reported results.
These results demonstrate that ZrO2 aerogel can be a powerful enrichment material for phosphoproteome study. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|