Effect of halogenated fluorescent compounds on bioluminescent reactions |
| |
Authors: | Tamara N Kirillova Marina A Gerasimova Elena V Nemtseva Nadezhda S Kudryasheva |
| |
Institution: | (1) Institute of Biophysics SB RAS, Akademgorodok 50, 660036 Krasnoyarsk, Russia;(2) Siberian Federal University, Svobodny Prospect 79, 660041 Krasnoyarsk, Russia; |
| |
Abstract: | The paper investigates an application of luminescent bioassays to monitor the toxicity of organic halides. Effects of xanthene
dyes (fluorescein, eosin Y, and erythrosin B), used as model compounds, on bioluminescent reactions of firefly Luciola mingrelica, marine bacteria Photobacterium leiognathi, and hydroid polyp Obelia longissima were studied. Dependence of bioluminescence quenching constants on the atomic weight of halogen substituents in dye molecules
was demonstrated. Bacterial bioluminescence was shown to be most sensitive to heavy halogen atoms involved in molecular structure;
hence, it is suitable for construction of sensors to monitor toxicity of halogenated compounds. Mechanisms of bioluminescence
quenching—energy transfer processes, collisional interactions, and enzyme–dye binding—were considered. Changes of bioluminescence
(BL) spectra in the presence of the dyes were analyzed. Interactions of the dyes with enzymes were studied using fluorescence
characteristics of the dyes in steady-state and time-resolved experiments. The dependences of fluorescence anisotropy of enzyme-bound
dyes, the average fluorescence lifetime, and the number of exponential components in fluorescence decay on the atomic weight
of halogen substituents were demonstrated. The results are discussed in terms of “dark effect of heavy halogen atom” in the
process of enzyme–dye binding; hydrophobic interactions were assumed to be responsible for the effect. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|