Microwave-assisted hydrolysis and extraction of tricyclic antidepressants from human hair |
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Authors: | Wietecha-Posłuszny Renata Garbacik Aneta Woźniakiewicz Michał Kościelniak Paweł |
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Institution: | (1) Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Jagiellonian University, 3 Ingardena St, 30-060 Krak?w, Poland;(2) Institute of Forensic Research, 9 Westerplatte St, 31-033 Krak?w, Poland |
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Abstract: | The objective of this research was to develop, optimize, and validate a modern, rapid method of preparation of human hair
samples, using microwave irradiation, for analysis of eight tricyclic antidepressants (TCADs): nordoxepin, nortriptyline,
imipramine, amitriptyline, doxepin, desipramine, clomipramine, and norclomipramine. It was based on simultaneous alkaline
hair microwave-assisted hydrolysis and microwave-assisted extraction (MAH–MAE). Extracts were analyzed by high-performance
liquid chromatography with diode-array detection (HPLC–DAD). A mixture of n-hexane and isoamyl alcohol (99:1, v/v) was used as extraction solvent and the process was performed at 60°C. Application of 1.0 mol L−1 NaOH and microwave irradiation for 40 min were found to be optimum for hair samples. Limits of detection ranged from 0.3
to 1.2 μg g−1 and LOQ from 0.9 to 4.0 μg g−1 for the different drugs. This enabled us to quantify them in hair samples within average therapeutic concentration ranges. |
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