A new method for the determination of free L-carnitine in serum samples based on high field single quantum coherence filtering 1H-NMR spectroscopy |
| |
| Authors: | Tsiafoulis Constantinos G Exarchou Vassiliki Tziova Polyxeni P Bairaktari Eleni Gerothanassis Ioannis P Troganis Anastassios N |
| |
| Affiliation: | (1) NMR Center, University of Ioannina, 45110 Ioannina, Greece;(2) University Hospital of Ioannina, Stavros Niarchos Ave., 45110 Ioannina, Greece;(3) Laboratory of Clinical Chemistry, Medical School, 45110 Ioannina, Greece;(4) Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece;(5) Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece; |
| |
| Abstract: | The rapid and accurate determination of specific metabolites present in biofluids is a very demanding task which is essential in both medicine and chemistry. l-carnitine (3-hydroxy-4-N-trimethylammonium butyrate) is an important metabolite which participates in a series of biological paths and therefore its determination is of diagnostic importance. A single quantum coherence filtering 1H NMR methodology was used for the accurate and rapid determination of l-carnitine in human serum samples. The methodology is based on spectral simplification, and specifically on the distinction of the N-methyl proton signal of l-carnitine that is greatly overlapped in the 1H-NMR spectrum of serum. The quantitative results provided by the proposed method are in excellent agreement with those obtained by the enzymatic method, which is widely used. The proposed method is rapid (~20 min of experimental time), selective, sensitive, and has good analytical characteristics (accuracy, reproducibility). Selected protein precipitation methods were also investigated and sample pretreatment with EtOH is suggested. |
| |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
