Effect of ligand structure of stationary phase of high performance hydrophobic interaction chromatography on renaturation efficiency of GuHCl-denaturedα-chymotrypsin
SHEN Yehua,WANG Haibo,BAI Quan & GENG Xindu Institute of Modern Separation Science,Shaanxi Key Laboratory of Modern Separation Science,Northwest University,Xi'an 710069,China
摘 要:
The renaturation of the denaturedα-chymotrypsin (α-Chy) with 1.7 mol·L-1 gua-nidine hydrochloride (GuHCI) by three kinds of stationary phase of high performance hydropho-bic interaction chromatography (STHIC) with a comparable hydrophobicity but different ligand structures was investigated. The obtained result indicates that the ligand structures of the three STHIC contribute to the renaturation efficiency ofα-Chy in the order of the end ligands PEG-600< phenyl group < tetrahydrofurfuryl alcohol (THFA).