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| Effect of ligand structure of stationary phase of high performance hydrophobic interaction chromatography on renaturation efficiency of GuHCl-denaturedα-chymotrypsin | |
| 作者姓名： | SHEN Yehua WANG Haibo BAI Quan & GENG Xindu Institute of Modern Separation Science Shaanxi Key Laboratory of Modern Separation Science Northwest University Xi'an China |
| 作者单位： | SHEN Yehua，WANG Haibo，BAI Quan & GENG Xindu Institute of Modern Separation Science，Shaanxi Key Laboratory of Modern Separation Science，Northwest University，Xi'an 710069，China |
| 摘 要： | The renaturation of the denaturedα-chymotrypsin (α-Chy) with 1.7 mol·L-1 gua-nidine hydrochloride (GuHCI) by three kinds of stationary phase of high performance hydropho-bic interaction chromatography (STHIC) with a comparable hydrophobicity but different ligand structures was investigated. The obtained result indicates that the ligand structures of the three STHIC contribute to the renaturation efficiency ofα-Chy in the order of the end ligands PEG-600< phenyl group < tetrahydrofurfuryl alcohol (THFA). |
| 关 键 词： | protein refolding renaturation α-chymotrypsin high performance hydrophobic interaction chromatography stationary phase |
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