Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers |
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Authors: | Schneider Robert Ader Christian Lange Adam Giller Karin Hornig Sönke Pongs Olaf Becker Stefan Baldus Marc |
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Affiliation: | Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 G?ttingen, Germany |
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Abstract: | We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel, KcsA-Kv1.3, in lipid bilayers. Sequential resonance assignments were obtained using a combination of (15)N- (13)C and (13)C- (13)C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K (+) channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function. |
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