鱼类免疫应答中的温度效应研究

本文通过对草鱼在不同温度 ( 10℃、 18℃、 26℃ )饲养条件下 ,注射细菌抗原 (嗜水产气单孢菌 ) ,用凝集抗体的检测方法测定其血清的凝集效价 ,研究不同水温下草鱼的体液免疫应答水平的差异 ,结果显示草鱼在 26℃ , 18℃ , 10℃三种温度下凝集抗体效价具有随时间推移 (抗原注射后 )而变化的规律 ,得出了草鱼在 18℃ , 26℃的温度范围内 ,温度越高 ,体液免疫应答的水平也越高 ,低温能抑制其体液免疫应答 .该文还研究了草鱼外周血中淋巴细胞 ,在不同温度 ( 10℃、 18℃、 26℃ )的体外培养中 ,分别受 PHA与SP A的刺激 ,经 3 H-Td R掺入法间接检测其细胞免疫应答 ,发现低温 ( 10℃、 18℃ )时 , T淋转受抑制 ,而 B淋转不受影响 ,反映出低温效应所引起的免疫抑制是与 T细胞免疫应答抑制有关 .

Research on Temperature Effect in Immune Response of Grass Carp(Ctenopharyngoden idellus Cuvier et Valencinnes).

Humoral immune responses o f g rass car p(Ctenopharyngoden idellus) in diffe rent temperaturew ere studied by testing the agg lutinate a ntibo dy titer after injecting A H( Aeromenas hy drophilic ) , the bacterial a ntigen. The regulation o f agglutina teantibody titer 's changing w as found in 10℃ , 18℃ , 26℃that the hig her th e tem pe ratur e w as, the faste r the agg lutinate antibody titer increased, and the hig herthe lev el of hum o ral im m une response w as, w hile low er tem pe ratur e w o uld inhibited fish 's im m une re-spo nding. And the peripher al blo od ly m phocy tes f rom accum ulated Grass Ca rp in different tem peratur e,10℃ , 18℃ a nd 26℃ , being stim ula ted by P HA and S PA, and being de tec ted by the m ag nitude of 3 H-Td Rinco rpo ra tio n, indica ted that T ly m phocytes transformation o f response w as inhibited at the low temperature ( 10℃ , 18℃ ) , but B lymphocytes no t. The results reflected that the temperature im m uno sup-pression resulted fro m prefential inhibitory effects o n T cell rather than B cell.