Sensitive ferrocene reagents for derivatization of amines for high-performance liquid chromatography with electrochemical detection |
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| Authors: | K Shimada T Oe M Tanaka T Nambara |
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| Institution: | Pharmaceutical Institute, Tohoku University, Sendai, Japan. |
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| Abstract: | Six reagents possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of amino compounds for their determination by high-performance liquid chromatography with electrochemical detection. The utility of these reagents was investigated employing phenethylamine as a model compound. Among these six, N-succinimidyl 3-ferrocenylpropionate was the best with respect to reactivity, stability and electrochemical properties. The developed method was applied to the determination of putrescine formed from ornithine by ornithine decarboxylase. |
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