An efficient reagent for 5′-azido oligonucleotide synthesis |
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Authors: | Jory Lietard |
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Affiliation: | Institut des Biomolécules Max Mousseron UMR 5247 CNRS-UM1-UM2, Université Montpellier II, Place E. Bataillon, CC1704, 34095 Montpellier Cedex 5, France |
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Abstract: | A new bromohexyl phosphoramidite was synthesized and used for the introduction of an azide function at the 5′-end of oligonucleotides after a treatment on solid support with sodium azide and sodium iodide. The corresponding 5′-azido oligonucleotide could be further used for ‘Click’ conjugation with alkyne derivatives or by Staudinger ligation. |
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Keywords: | Azidation 1,3-Dipolar cycloaddition Click chemistry Staudinger ligation |
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