Analysis of novel imidazoles from isolated perfused rabbit heart by two high-performance liquid chromatographic methods. |
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Authors: | A O'Dowd J J O'Dowd J J O'Dowd N MacFarlane H Abe D J Miller |
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Affiliation: | Institute of Physiology, Glasgow University, Scotland, UK. |
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Abstract: | The paper reports two analytical high-performance liquid chromatographic methods to detect and quantify cardiac-derived histidyl derivatives. Method A relies on relative hydrophobicities as a basis of separation. Method B is an ion-pairing method in which the compounds are eluted in an entirely different order. Fractions collected from method A have been co-eluted in admixture in method B with authentic reference compounds. Thus the existence of the following imidazole ring-containing compounds derived from heart have been confirmed: N-acetylhistidine, N-acetyl-1-methylhistidine, N-acetylcarnosine, N-acetylhomocarnosine, homocarnosine, anserine, carnosine, balenine. Compounds were found in both tissue samples and perfusates. |
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