| CLONING OF cDNA CODING FOR CARBAMYL PHOSPHATE SYNTHETASE I AND CHANGES IN LEVELS OF CPS_1 mRNA DURING HEPATOCARCINOGENESIS |
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| 作者姓名： | 吴石君 李士谔 张海澜 罗坚 于跃 |
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| 作者单位： | Faculty of Basic Medical Sciences，Peking Union Medical College，Institute of Basic Medical Sciences.Chinese Academy of Medical Sciences. Beijing，Faculty of Basic Medical Sciences，Peking Union Medical College，Institute of Basic Medical Sciences.Chinese Academy of Medical Sciences. Beijing，Faculty of Basic Medical Sciences，Peking Union Medical College，Institute of Basic Medical Sciences.Chinese Academy of Medical Sciences. Beijing，Faculty of Basic Medical Sciences，Peking Union Medical College，Institute of Basic Medical Sciences.Chinese Academy of Medical Sciences. Beijing，Faculty of Basic Medical Sciences，Peking Union Medical College，Institute of Basic Medical Sciences.Chinese Academy of Medical Sciences. Beijing |
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| 摘 要： | cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insertcomplementary to the mRNA for CPS_1 followed by hybrid-selected translation screening. The length ofthe insert CPS_1 cDNA was approximately 800 base pairs. Using this cDNA as a probe, it was foundby dot-blot analysis of the total RNAs and poly(A)~+-RNAs isolated from rat livers with different path-ological lesions induced by diethylnitrosamine that the levels of CPS_1 mRNA were decreased, the de-crease being correlated with the malignancy of hepatocytes during carcinogenesis.
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