| 牛血清白蛋白与两种金属配合物的作用研究 |
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| 引用本文： | 刘海萍,王兴明,戴亚堂,宋绵新.牛血清白蛋白与两种金属配合物的作用研究[J].无机化学学报,2005,21(9):1422-1426. |
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| 作者姓名： | 刘海萍 王兴明 戴亚堂 宋绵新 |
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| 作者单位： | 西南科技大学材料科学与工程学院应用化学研究所,绵阳,621010 |
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| 基金项目： | 国家自然科学基金资助项目(No.40072020)，四川省教育厅自然科学基金重点资助项目(No.2000-A56) |
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| 摘 要： | The interaction of Methylthymol Blue(MTB)-Zinc(Ⅱ) compound and Alizarin Red S(ARS)-Aluminum(Ⅲ) compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn(Ⅱ)-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn(Ⅱ) complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained: the apparent molar absorptivity of MTB-Zn(Ⅱ) with BSA was ε=2.20×104 L·mol-1·cm-1. Conditional proportion were defined, nMTB∶nZn(Ⅱ)∶nBSA=2∶2∶1; condition combination constant, K=2.07×1010. Combination proportion were defined, nARS∶nAl(Ⅲ)∶nBSA=6∶4∶1. Condition equilibrium constant of reaction of ARS-Al(Ⅲ) with BSA was K=8.80×108. The apparent molar absorptivity of ε=2.65×104 L·mol-1·cm-1. It is suggested that combination between BSA and MTB-Zn(Ⅲ) is due to coordination force. That combination between BSA and ARS-Al(Ⅲ) is due to the coordination bond and electrostatic force.
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| 关 键 词： | UV-Vis光谱法 甲基百里酚蓝-Zn(Ⅱ)-配合物 牛血清白蛋白 茜素红S-Al(Ⅲ)-配合物 |
| 文章编号： | 1001-4861(2005)09-1422-05 |
| 收稿时间： | 2005-03-28 |
| 修稿时间： | 2005-05-23 |
Interaction of Bovine Serum Albumin with Twain Metal Complexes |
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| LIU Hai-Ping,WANG Xing-Ming,DAI Ya-Tang and SONG Mian-Xin.Interaction of Bovine Serum Albumin with Twain Metal Complexes[J].Chinese Journal of Inorganic Chemistry,2005,21(9):1422-1426. |
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| Authors: | LIU Hai-Ping WANG Xing-Ming DAI Ya-Tang and SONG Mian-Xin |
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| Institution: | Institute of Applied Chemistry, Southwest University of Science and Technology, Mianyang 621010,Institute of Applied Chemistry, Southwest University of Science and Technology, Mianyang 621010,Institute of Applied Chemistry, Southwest University of Science and Technology, Mianyang 621010 and Institute of Applied Chemistry, Southwest University of Science and Technology, Mianyang 621010 |
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| Abstract: | |
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| Keywords: | UV-Vis spectroscopy methylthymol blue-zinc! complex bovine serum albumin alizarin red S-aluminum" complex |
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