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Structure of hydroxycinnamic acid derivatives established by high-perfomance liquid chromatography with photodiode-array detection
Authors:L Bengoechea  T Hernández  C Quesada  B Bartolomé  I Estrella  C Gómez-Cordovés
Institution:(1) Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain;(2) Present address: Department of Food Molecular Biochemistry, Institute of Food Research, Norwich Research Park, NR4 7UA Colney, Norwich, UK
Abstract:Summary The present paper describes the development of a method for the differentiation of hydroxycinnamic acid derivatives by HPLC with photodiode-array detection. Furthermore spectral data of the compounds under investigation are given. Whereas p-coumaric acid derivatives are distinguishable from caffeic and ferulic acid derivatives by the positions of their spectrum maxima and their convexity interval value, it is not possible to distinguish between caffeic and ferulic acid derivatives because of overlapping spectrum maxima and convexity interval values.
Keywords:Column liquid chromatography  Hydroxycinnamic derivatives  Photodiode-array detection (DAD)
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