| 桃褐腐病菌角质酶基因的克隆与原核表达 |
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| 引用本文： | 麻莹,责祎旦·加帕尔,葛喜珍,田平芳.桃褐腐病菌角质酶基因的克隆与原核表达[J].北京化工大学学报(自然科学版),2013,40(2):61-64. |
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| 作者姓名： | 麻莹 责祎旦·加帕尔 葛喜珍 田平芳 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京联合大学生物化学工程学院,北京,100023 |
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| 摘 要： | 采用PCR法克隆了桃褐腐病菌的角质酶基因cutl，构建了诱导型表达载体pET28a-cutl，转化大肠杆菌E. coli(BL21)，获得重组菌pET28a-cutl/ BL21。经异丙基-β-D-硫代吡喃半乳糖苷(IPTG)诱导，该重组菌角质酶的表达量约为对照菌的1.69倍，酶活约为对照菌的1.8倍，粗酶液的酶活可达40.33U/mL。
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| 关 键 词： | 桃褐腐病菌 角质酶 原核表达 |
| 收稿时间： | 2012-05-28 |
Cloning and prokaryotic expression of the cutinase gene from Monilinia fructicola |
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| MA Ying
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Zeyidan JIAPAER
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GE XiZhen
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TIAN PingFang.Cloning and prokaryotic expression of the cutinase gene from Monilinia fructicola[J].Journal of Beijing University of Chemical Technology,2013,40(2):61-64. |
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| Authors: | MA Ying Zeyidan JIAPAER GE XiZhen TIAN PingFang |
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| Institution: | 1.College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029;2. Biochemical Engineering College of Beijing Union University, Beijing 100023, China |
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| Abstract: | The cutinase gene cutl has been cloned by a polymerase chain reaction (PCR) from Monolinia fructicola. After ligation to vector pET28a and transformation into E. coli (BL21), a recombinant strain pET28a cutl/BL21 was acquired. Upon isopropylthio-β-galactoside (IPTG) induction, this recombinant strain produced 1.69 times as much cutinase as the control strain. The maximum cutinase activity of the fermentation broth was 40.33U/mL, nearly 1.8 times that of the control strain. |
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