Simultaneous determination of uric acid, xanthine and hypoxanthine with an electrochemically pretreated carbon paste electrode |
| |
Authors: | X. Cai K. Kalcher C. Neuhold |
| |
Affiliation: | (1) College of Science and Technology, Hainan University, China;(2) Institut für Analytische Chemie, Karl-Franzens Universität Graz, Universitätsplatz 1, A-8010 Graz, Austria |
| |
Abstract: | A simple method is presented for the simultaneous differential pulse voltammetric determination of uric acid, xanthine and hypoxanthine. It is based on the improved current responses of the three analytes at carbon paste electrodes polarized in a dilute alkaline medium (0.002 mol/l NaOH, 0.1 mol/l NaClO4) at 1.3 V vs. SCE for a short time. Compared with the methods reported in the literature, this procedure has a much wider linear range (2 to 3 orders of magnitude in concentration), lower detection limits (5 to 10 g l–1) and less interference by ascorbic acid. The electrochemical responses were found to be dependent on the pre-anodization potential and the time imposed on the electrodes as well as on the alkalinity of the supporting electrolyte. The proposed procedure was used to determine uric acid, xanthine and hypoxanthine in human urine without any preliminary treatment. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|