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Synthesis of ratiometric fluorescent nanoparticles for sensing oxygen |
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| Authors: | Xiao-Hui Wang Hong-Shang Peng Zhuo Chang Ling-Ling Hou Fang-Tian You Feng Teng Hong-Wei Song Biao Dong |
| Institution: | 1. Key Laboratory of Luminescence and Optical Information, Ministry of Education, Institute of Optoelectronic Technology, Beijing Jiaotong University, Beijing, 100044, China 2. College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing, 100044, China 3. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China |
| Abstract: | A facile reprecipitation-encapsulation method is used for the preparation of ratiometric fluorescent nanoparticles (NPs) for sensing intracellular oxygen. The surface of the NPs is modified in-situ with poly-L-lysine, which renders good biocompatibility and enables easy internalization into living cells. The sensor NPs contain a red fluorescent probe whose fluorescence is sensitive to oxygen with a quenching response of 77 % on going from nitrogen saturation to oxygen saturation, and a reference dye giving a green signal that acts as an oxygen-independent reference. The ratio of the two emissions serves as the analytical information and is sensitive to dissolved oxygen in the 0–43?ppm concentration range. When incorporated into cells, the ratio of the signals increases by 400?% on going from oxygen-saturated to oxygen-free environment. Dissolved oxygen could be visually detected using the ratiometric nanoparticles. Under single-wavelength excitation, red fluorescence is highly sensitive to oxygen, whereas green fluorescence keeps constantly. |
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