| 活性蓝F3GA固载的壳聚糖微球对过氧化氢酶的吸附 |
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| 引用本文： | 申屠静灵,贾之慎,高慧. 活性蓝F3GA固载的壳聚糖微球对过氧化氢酶的吸附[J]. 浙江大学学报(理学版), 2005, 32(6): 674-677,684 |
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| 作者姓名： | 申屠静灵 贾之慎 高慧 |
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| 作者单位： | 1. 浙江大学,化学系,浙江,杭州,310027
2. 浙江赞成科技有限公司,浙江,杭州,310009 |
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| 摘 要： | 将反相悬浮交联法制备的壳聚糖微球树脂与三嗪染料活性蓝F3GA(Cibacron Blue F3GA)反应,制得亲和色谱填料,并采用扫描电镜、红外光谱、染料泄漏率测定等对此填料进行鉴定与表征.该填料具有良好的色谱性能,在pH为7.0的磷酸盐缓冲液体系下,对过氧化氢酶的饱和吸附容量为24.6 mg/g,改变pH值及离子强度对结合量有明显影响.
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| 关 键 词： | 活性蓝F3GA 过氧化氢酶 亲和色谱 壳聚糖微球树脂 |
| 文章编号： | 1008-9497（2005）06-674-04 |
| 收稿时间： | 2004-10-15 |
| 修稿时间： | 2004-10-15 |
Catalase adsorption onto cibacron blue F3GA-attached chitosan microspheres |
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| SHENTU Jing-ling,JIA Zhi-shen,GAO Hui. Catalase adsorption onto cibacron blue F3GA-attached chitosan microspheres[J]. Journal of Zhejiang University(Sciences Edition), 2005, 32(6): 674-677,684 |
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| Authors: | SHENTU Jing-ling JIA Zhi-shen GAO Hui |
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| Affiliation: | 1. Department of Chemistry, Zhejiang University, Hangzhou 310027 China; 2. Zhejiang Approval Technology Development Limited Corporation, Hangzhou 310009, China |
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| Abstract: | Chitosan microsphere was prepared by phase-inversion method as the support matrices. Cibacron blue F3GA was covalently attached onto the chitosan microspheres. The chitosan microsphere structure was characterized by scanning electron microscopy. These dye-carrying chitosan microspheres were used in the catalase adsorption studies. The non-specific adsorption value was 2. 758 mg/g. Cibacron blue F3GA attachment increased the adsorption capacity up to 24.6 mg/g. Langmuir adsorption model was found to be applicable in interpreting catalase adsorption by Cibacron blue F3GA-attached chitosan microspheres. The effect of pH value and ionic strength on cata- lase adsorption were investigated. It showed that F3GA attached chitosan microspheres could be applied in adsorption and purification of catalase without significant denaturation. |
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| Keywords: | Cibacron blue F3GA catalase affinity chromatography chitosan microspheres |
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