| Real—time Analysis of the Interaction between Calmodulin and Melittin by SPR Spectroscopy |
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| 作者姓名： | Wei Guo LI Xiao Qiang CUI Xiu Rong YANG Da Qing ZHAO* Jia Zuan NI Key Laboratory of Rare Earth Chemistry and Physics Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun State Key Laboratory of Electroana |
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| 作者单位： | Wei Guo LI1，Xiao Qiang CUI2，Xiu Rong YANG2，Da Qing ZHAO1*，Jia Zuan NI1 1 Key Laboratory of Rare Earth Chemistry and Physics，Changchun Institute of Applied Chemistry，Chinese Academy of Sciences，Changchun 130022 2 State Key Laboratory of Electroana |
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| 基金项目： | The authors thank the NNSF for financial support of this work (No. 29890280). |
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| 摘 要： | Surface Plasmon Resonance (SPR) is a new technique for the biomolecular interaction analysis developed from 1990. Compared with traditional methods of analysis, SPR has its obvious advantages: fast-speed, no-labeling, real-time and micro sample requiring, etc.. In this case, it has been applied extensively to the studies in life research1,2. Calmodulin (CaM) is a ubiquitous calcium-binding protein in eucaryotic cells and serves as a multifunctional regulator in variety of cellular proces…
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| 关 键 词： | 实时分析 调钙蛋白 调钙素 蜜蜂毒素 表面等离子体共振光谱学 |
Real-time Analysis of the Interaction between Calmodulin and Melittin by SPR Spectroscopy |
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| Wei Guo LI,Xiao Qiang CUI,Xiu Rong YANG,Da Qing ZHAO*,Jia Zuan NI Key Laboratory of Rare Earth Chemistry and Physics,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun State Key Laboratory of Electroana.Real-time Analysis of the Interaction between Calmodulin and Melittin by SPR Spectroscopy[J].Chinese Chemical Letters,2002,13(2):165-166. |
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| Authors: | Wei Guo LI Xiao Qiang CUI Xiu Rong YANG Da Qing ZHAO Jia Zuan NI |
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| Abstract: | The dynamic interaction process of calmodulin with an immobilized peptide melittin was investigated in real time by surface plasmon resonance spectroscopy, and dissociation constant of the complex was calculated to be 3.3710-6 mol/L. |
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| Keywords: | Calmodulin melittin surface plasmon resonance |
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